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A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude.

ABSTRACT: The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

SUBMITTER: Acharya AP

PROVIDER: S-EPMC4011665 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude.

Acharya Abhinav P AP Nafisi Parsa M PM Gardner Austin A Mackay Joanna L JL Kundu Kousik K Kumar Sanjay S Murthy Niren N

Chemical communications (Cambridge, England) 20131101 88

The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude. ...[more]

PMID: 24071916

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