Unknown

Dataset Information

0

A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude.

ABSTRACT: The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

SUBMITTER: Acharya AP 

PROVIDER: S-EPMC4011665 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude.

Acharya Abhinav P AP   Nafisi Parsa M PM   Gardner Austin A   Mackay Joanna L JL   Kundu Kousik K   Kumar Sanjay S   Murthy Niren N  

Chemical communications (Cambridge, England) 20131101 88

The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude. ...[more]

Similar Datasets

Unknown In situ Stiffness Adjustment of AFM Probes by Two Orders of Magnitude.
| S-EPMC4851037 | biostudies-literature
Unknown Two-photon-like microscopy with orders-of-magnitude lower illumination intensity via two-step fluorescence.
| S-EPMC4559865 | biostudies-literature
Unknown Antimonene with two-orders-of-magnitude improved stability for high-performance cancer theranostics.
| S-EPMC6520929 | biostudies-literature
Unknown Effort increases sensitivity to reward and loss magnitude in the human brain.
| S-EPMC3980794 | biostudies-other
Unknown Synergy between Cyclase-associated protein and Cofilin accelerates actin filament depolymerization by two orders of magnitude.
| S-EPMC6876572 | biostudies-literature
Unknown LC/ESI-MS/MS detection of FAs by charge reversal derivatization with more than four orders of magnitude improvement in sensitivity.
| S-EPMC3826698 | biostudies-literature
Unknown A ratiometric two-photon fluorescent probe for hydrazine and its applications.
| S-EPMC4612371 | biostudies-literature
Unknown An NMR-based scoring function improves the accuracy of binding pose predictions by docking by two orders of magnitude.
| S-EPMC3266494 | biostudies-literature
Unknown Two Orders of Magnitude Boost in the Detection Limit of Droplet-Based Micro-Magnetofluidics with Planar Hall Effect Sensors.
| S-EPMC7439703 | biostudies-literature
Unknown Simple and Rapid Endotoxin Recognition Using a Dipicolylamine-Modified Fluorescent Probe with Picomolar-Order Sensitivity.
| S-EPMC9330845 | biostudies-literature