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A cephalosporin-chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude.

ABSTRACT: The expression of beta-lactamases in bacteria is a central cause of drug resistance. In this report, we present a beta-lactamase chemiluminescent probe, termed CCP, which can for the first time detect beta-lactamase activity via chemiluminescence and can detect beta lactamase with a sensitivity that is 4-orders of magnitude higher than the commercially available fluorescent lactamase substrate fluorocillin.

SUBMITTER: Maity S

PROVIDER: S-EPMC7666973 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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A cephalosporin-chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude.

Maity Santanu S Wang Xiaojian X Das Subhamoy S He Maomao M Riley Lee W LW Murthy Niren N

Chemical communications (Cambridge, England) 20200226 24

The expression of beta-lactamases in bacteria is a central cause of drug resistance. In this report, we present a beta-lactamase chemiluminescent probe, termed CCP, which can for the first time detect beta-lactamase activity via chemiluminescence and can detect beta lactamase with a sensitivity that is 4-orders of magnitude higher than the commercially available fluorescent lactamase substrate fluorocillin. ...[more]

PMID: 32101196

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