Ibrolipim increases ABCA1/G1 expression by the LXR? signaling pathway in THP-1 macrophage-derived foam cells.

ABSTRACT: To determine the effects and potential mechanisms of ibrolipim on ATP-binding membrane cassette transporter A-1 (ABCA1) and ATP-binding membrane cassette transporter G-1 (ABCG1) expression from human macrophage foam cells, which may play a critical role in atherogenesis.Human THP-1 cells pre-incubated with ox-LDL served as foam cell models. Specific mRNA was quantified using real-time RT-PCR and protein expression using Western blotting. Cellular cholesterol handling was studied using cholesterol efflux experiments and high performance liquid chromatography assays.Ibrolipim 5 and 50 ?mol/L significantly increased cholesterol efflux from THP-1 macrophage-derived foam cells to apoA-I or HDL. Moreover, it upregulated the expression of ABCA1 and ABCG1. In addition, LXR? was also upregulated by the ibrolipim treatment. In addition, LXR? small interfering RNA completely abolished the promotion effect that was induced by ibrolipim.Ibrolipim increased ABCA1 and ABCG1 expression and promoted cholesterol efflux, which was mediated by the LXR? signaling pathway.

SUBMITTER: Chen SG 

PROVIDER: S-EPMC4012897 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature