Mechanisms of nanoparticle-mediated siRNA transfection by melittin-derived peptides.
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ABSTRACT:
SUBMITTER: Hou KK
PROVIDER: S-EPMC4013830 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Publications
Mechanisms of nanoparticle-mediated siRNA transfection by melittin-derived peptides.
Hou Kirk K KK Pan Hua H Ratner Lee L Schlesinger Paul H PH Wickline Samuel A SA
ACS nano 20130926 10
Traditional peptide-mediated siRNA transfection via peptide transduction domains exhibits limited cytoplasmic delivery of siRNA due to endosomal entrapment. This work overcomes these limitations with the use of membrane-destabilizing peptides derived from melittin for the knockdown of NFkB signaling in a model of adult T-cell leukemia/lymphoma. While the mechanism of siRNA delivery into the cytoplasmic compartment by peptide transduction domains has not been well studied, our analysis of melitti ...[more]
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