Enhancing oligodendrocyte differentiation by transient transcription activation via DNA nanoparticle-mediated transfection.

ABSTRACT: Current approaches to derive oligodendrocytes from human pluripotent stem cells (hPSCs) need extended exposure of hPSCs to growth factors and small molecules, which limits their clinical application because of the lengthy culture time required and low generation efficiency of myelinating oligodendrocytes. Compared to extrinsic growth factors and molecules, oligodendrocyte differentiation and maturation can be more effectively modulated by regulation of the cell transcription network. In the developing central nervous system (CNS), two basic helix-loop-helix transcription factors, Olig1 and Olig2, are decisive in oligodendrocyte differentiation and maturation. Olig2 plays a critical role in the specification of oligodendrocytes and Olig1 is crucial in promoting oligodendrocyte maturation. R

SUBMITTER: Li X 

PROVIDER: S-EPMC5485910 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature