Ontology highlight
ABSTRACT:
SUBMITTER: Bagert JD
PROVIDER: S-EPMC4014290 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Bagert John D JD Xie Yushu J YJ Sweredoski Michael J MJ Qi Yutao Y Hess Sonja S Schuman Erin M EM Tirrell David A DA
Molecular & cellular proteomics : MCP 20140221 5
An approach to proteomic analysis that combines bioorthogonal noncanonical amino acid tagging (BONCAT) and pulsed stable isotope labeling with amino acids in cell culture (pSILAC) provides accurate quantitative information about rates of cellular protein synthesis on time scales of minutes. The method is capable of quantifying 1400 proteins produced by HeLa cells during a 30 min interval, a time scale that is inaccessible to isotope labeling techniques alone. Potential artifacts in protein quant ...[more]