Fabrication of enzyme-degradable and size-controlled protein nanowires using single particle nano-fabrication technique.

ABSTRACT: Protein nanowires exhibiting specific biological activities hold promise for interacting with living cells and controlling and predicting biological responses such as apoptosis, endocytosis and cell adhesion. Here we report the result of the interaction of a single high-energy charged particle with protein molecules, giving size-controlled protein nanowires with an ultra-high aspect ratio of over 1,000. Degradation of the human serum albumin nanowires was examined using trypsin. The biotinylated human serum albumin nanowires bound avidin, demonstrating the high affinity of the nanowires. Human serum albumin-avidin hybrid nanowires were also fabricated from a solid state mixture and exhibited good mechanical strength in phosphate-buffered saline. The biotinylated human serum albumin nanowir

SUBMITTER: Omichi M 

PROVIDER: S-EPMC4015332 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature