Ontology highlight
ABSTRACT:
SUBMITTER: Lin C
PROVIDER: S-EPMC5632796 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Lin Chuhong C Kätelhön Enno E Sepunaru Lior L Compton Richard G RG
Chemical science 20170719 9
To evaluate the possible detection of single enzyme activity <i>via</i> electrochemical methods, a combined finite difference and random walk simulation is used to model individual enzyme-electrode collisions where such events are monitored amperometrically <i>via</i> the measurement of products formed by the enzyme in solution. It is found that the observed signal is highly sensitive to both the enzyme turnover number, the size of the electrode and the bandwidth of the electronics. Taking singl ...[more]