Ontology highlight
ABSTRACT:
SUBMITTER: Krueger AT
PROVIDER: S-EPMC4018821 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Krueger Andrew T AT Kroll Carsten C Sanchez Edgar E Griffith Linda G LG Imperiali Barbara B
Angewandte Chemie (International ed. in English) 20140131 10
Described is the development and application of a versatile semisynthetic strategy, based on a combination of sortase-mediated coupling and tetrazine ligation chemistry, which can be exploited for the efficient incorporation of tunable functionality into chimeric recombinant proteins. To demonstrate the scope of the method, the assembly of a set of bivalent ligands, which integrate members of the epidermal growth factor (EGF) ligand family, is described. By using a series of bivalent EGFs with v ...[more]