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Cellular imaging of deep organ using two-photon Bessel light-sheet nonlinear structured illumination microscopy.

ABSTRACT: In vivo fluorescent cellular imaging of deep internal organs is highly challenging, because the excitation needs to penetrate through strong scattering tissue and the emission signal is degraded significantly by photon diffusion induced by tissue-scattering. We report that by combining two-photon Bessel light-sheet microscopy with nonlinear structured illumination microscopy (SIM), live samples up to 600 microns wide can be imaged by light-sheet microscopy with 500 microns penetration depth, and diffused background in deep tissue light-sheet imaging can be reduced to obtain clear images at cellular resolution in depth beyond 200 microns. We demonstrate in vivo two-color imaging of pronephric glomeruli and vasculature of zebrafish kidney, whose cellular structures located at the center of the fish body are revealed in high clarity by two-color two-photon Bessel light-sheet SIM.

SUBMITTER: Zhao M

PROVIDER: S-EPMC4026892 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Cellular imaging of deep organ using two-photon Bessel light-sheet nonlinear structured illumination microscopy.

Zhao Ming M Zhang Han H Li Yu Y Ashok Amit A Liang Rongguang R Zhou Weibin W Peng Leilei L

Biomedical optics express 20140331 5

In vivo fluorescent cellular imaging of deep internal organs is highly challenging, because the excitation needs to penetrate through strong scattering tissue and the emission signal is degraded significantly by photon diffusion induced by tissue-scattering. We report that by combining two-photon Bessel light-sheet microscopy with nonlinear structured illumination microscopy (SIM), live samples up to 600 microns wide can be imaged by light-sheet microscopy with 500 microns penetration depth, and ...[more]

PMID: 24876996

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