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Imaging neural events in zebrafish larvae with linear structured illumination light sheet fluorescence microscopy.

ABSTRACT: Light sheet fluorescence microscopy (LSFM) is a powerful tool for investigating model organisms including zebrafish. However, due to scattering and refractive index variations within the sample, the resulting image often suffers from low contrast. Structured illumination (SI) has been combined with scanned LSFM to remove out-of-focus and scattered light using square-law detection. Here, we demonstrate that the combination of LSFM with linear reconstruction SI can further increase resolution and contrast in the vertical and axial directions compared to the widely adopted root-mean square reconstruction method while using the same input images. We apply this approach to imaging neural activity in 7-day postfertilization zebrafish larvae. We imaged two-dimensional sections of the zebrafish central nervous system in two colors at an effective frame rate of 7 frames per second.

SUBMITTER: Liu Y

PROVIDER: S-EPMC6400141 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Imaging neural events in zebrafish larvae with linear structured illumination light sheet fluorescence microscopy.

Liu Yang Y Dale Savannah S Ball Rebecca R VanLeuven Ariel J AJ Sornborger Andrew A Lauderdale James D JD Kner Peter P

Neurophotonics 20190101 1

Light sheet fluorescence microscopy (LSFM) is a powerful tool for investigating model organisms including zebrafish. However, due to scattering and refractive index variations within the sample, the resulting image often suffers from low contrast. Structured illumination (SI) has been combined with scanned LSFM to remove out-of-focus and scattered light using square-law detection. Here, we demonstrate that the combination of LSFM with linear reconstruction SI can further increase resolution and ...[more]

PMID: 30854407

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