Dataset Information

Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation.

ABSTRACT:

Background

We previously found that the low frequency magnetic fields (LF-MF) inhibited gastric and lung cancer cell growth. We suppose that exposure to LF-MF may modulate immune function so as to inhibit tumor. We here investigated whether LF-MF can inhibit the proliferation and metastasis of melanoma and influence immune function.

Methods

The effect of MF on the proliferation, cell cycle and ultrastracture of B16-F10 in vitro was detected by cell counting Kit-8 assay, flow cytometry, and transmission electron microscopy. Lung metastasis mice were prepared by injection of 2 × 105 B16-F10 melanoma cells into the tail vein in C57BL/6 mice. The mice were then exposed to an LF-MF (0.4 T, 7.5 Hz) for 43 days. Survival rate, tumor markers and the innate and adaptive immune parameters were measured.

Results

The growth of B16-F10 cells was inhibited after exposure to the LF-MF. The inhibition was related to induction of cell cycle arrest and decomposition of chromatins. Moreover, the LF-MF prolonged the mouse survival rate and inhibited the proliferation of B16-F10 in melanoma metastasis mice model. Furthermore, the LF-MF modulated the immune response via regulation of immune cells and cytokine production. In addition, the number of Treg cells was decreased in mice with the LF-MF exposure, while the numbers of T cells as well as dendritic cells were significantly increased.

Conclusion

LF-MF inhibited the growth and metastasis of melanoma cancer cells and improved immune function of tumor-bearing mice. This suggests that the inhibition may be attributed to modulation of LF-MF on immune function and LF-MF may be a potential therapy for treatment of melanoma.

SUBMITTER: Nie Y

PROVIDER: S-EPMC4029221 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Publications

Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation.

Nie Yunzhong Y Du Leilei L Mou Yongbin Y Xu Zhenjun Z Weng Leihua L Du Youwei Y Zhu Yanan Y Hou Yayi Y Wang Tingting T

BMC cancer 20131206

<h4>Background</h4>We previously found that the low frequency magnetic fields (LF-MF) inhibited gastric and lung cancer cell growth. We suppose that exposure to LF-MF may modulate immune function so as to inhibit tumor. We here investigated whether LF-MF can inhibit the proliferation and metastasis of melanoma and influence immune function.<h4>Methods</h4>The effect of MF on the proliferation, cell cycle and ultrastracture of B16-F10 in vitro was detected by cell counting Kit-8 assay, flow cytom ...[more]

PMID: 24314291

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Dataset Information

Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation.

Background

Methods

Results

Conclusion

Publications

Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation.

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