Unknown

Dataset Information

0

Distribution of D-3-aminoisobutyrate-pyruvate aminotransferase in the rat brain.


ABSTRACT:

Background

D-3-aminoisobutyrate, an intermediary product of thymine, is converted to 2-methyl-3-oxopropanoate using pyruvate as an amino acceptor by D-3-aminoisobutyrate-pyruvate aminotransferase (D-AIB AT; EC 2.6.1.40). A large amount of D-AIB AT is distributed in the kidney and liver; however, small amounts are found in the brain. Recently, D-AIB AT was reported to metabolize asymmetric dimethylarginine (ADMA) in vivo and was suggested to be an important enzyme for nitric oxide metabolism because ADMA is a competitive inhibitor for nitric oxide synthase. In this study, we examined the distribution of D-AIB AT in the rat brain further to understand its role. We measured D-AIB AT mRNA and protein expression using quantitative RT-PCR and Western blotting, and monitored its distribution using immunohistochemical staining.

Results

D-AIB AT was distributed throughout the brain, with high expression in the cortex and hippocampus. Immunohistochemical staining revealed that D-AIB AT was highly expressed in the retrosplenial cortex and in hippocampal neurons.

Conclusion

Our results suggest that D-AIB AT is distributed in the examined- just the regions and may play an important role there.

SUBMITTER: Abe M 

PROVIDER: S-EPMC4030283 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Distribution of D-3-aminoisobutyrate-pyruvate aminotransferase in the rat brain.

Abe Masao M   Ochi Shinichiro S   Mori Yoko Y   Yamazaki Kiyohiro K   Ishimaru Takashi T   Yoshino Yuta Y   Fukuhara Ryuji R   Tanimukai Satoshi S   Matsuda Seiji S   Ueno Shu-Ichi S  

BMC neuroscience 20140427


<h4>Background</h4>D-3-aminoisobutyrate, an intermediary product of thymine, is converted to 2-methyl-3-oxopropanoate using pyruvate as an amino acceptor by D-3-aminoisobutyrate-pyruvate aminotransferase (D-AIB AT; EC 2.6.1.40). A large amount of D-AIB AT is distributed in the kidney and liver; however, small amounts are found in the brain. Recently, D-AIB AT was reported to metabolize asymmetric dimethylarginine (ADMA) in vivo and was suggested to be an important enzyme for nitric oxide metabol  ...[more]

Similar Datasets

| S-EPMC1163905 | biostudies-other
| S-EPMC1158134 | biostudies-other
| S-EPMC1161423 | biostudies-other
| S-EPMC1164486 | biostudies-other
| S-EPMC1482817 | biostudies-literature
| S-EPMC1164159 | biostudies-other
| S-EPMC4409981 | biostudies-literature
| S-EPMC1186129 | biostudies-other
| S-EPMC1172807 | biostudies-other
| S-EPMC2915787 | biostudies-literature