Ontology highlight
ABSTRACT:
SUBMITTER: Yu XW
PROVIDER: S-EPMC4031936 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Yu Xiao-Wei XW Zhu Shan-Shan SS Xiao Rong R Xu Yan Y
Journal of lipid research 20140326 6
In an effort to explore the feasibility of converting a lipase into an esterase by modifying the lid region, we designed and characterized two novel Rhizopus chinensis lipase variants by lid swapping. The substrate specificity of an R. chinensis lipase was successfully modified toward water-soluble substrates, that is, turned into an esterase, by replacing the hydrophobic lid with a hydrophilic lid from ferulic acid esterase from Aspergillus niger Meanwhile, as a comparison, the lid of R. chinen ...[more]