Ontology highlight
ABSTRACT:
SUBMITTER: DeWitt MA
PROVIDER: S-EPMC4033606 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
DeWitt Mark A MA Chang Amy Y AY Combs Peter A PA Yildiz Ahmet A
Science (New York, N.Y.) 20111208 6065
Cytoplasmic dynein is a homodimeric AAA+ motor that transports a multitude of cargos toward the microtubule minus end. How the two catalytic head domains interact and move relative to each other during processive movement is unclear. Here, we tracked the relative positions of both heads with nanometer precision and directly observed the heads moving independently along the microtubule. The heads remained widely separated, and their stepping behavior varied as a function of interhead separation. ...[more]