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Insulin regulates glucose consumption and lactate production through reactive oxygen species and pyruvate kinase M2.


ABSTRACT: Although insulin is known to regulate glucose metabolism and closely associate with liver cancer, the molecular mechanisms still remain to be elucidated. In this study, we attempt to understand the mechanism of insulin in promotion of liver cancer metabolism. We found that insulin increased pyruvate kinase M2 (PKM2) expression through reactive oxygen species (ROS) for regulating glucose consumption and lactate production, key process of glycolysis in hepatocellular carcinoma HepG2 and Bel7402 cells. Interestingly, insulin-induced ROS was found responsible for the suppression of miR-145 and miR-128, and forced expression of either miR-145 or miR-128 was sufficient to abolish insulin-induced PKM2 expression. Furthermore, the knockdown of PKM2 expression also inhibited cancer cell growth and insulin-induced glucose consumption and lactate production, suggesting that PKM2 is a functional downstream effecter of insulin. Taken together, this study would provide a new insight into the mechanism of insulin-induced glycolysis.

SUBMITTER: Li Q 

PROVIDER: S-EPMC4034658 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Insulin regulates glucose consumption and lactate production through reactive oxygen species and pyruvate kinase M2.

Li Qi Q   Liu Xue X   Yin Yu Y   Zheng Ji-Tai JT   Jiang Cheng-Fei CF   Wang Jing J   Shen Hua H   Li Chong-Yong CY   Wang Min M   Liu Ling-Zhi LZ   Jiang Bing-Hua BH  

Oxidative medicine and cellular longevity 20140508


Although insulin is known to regulate glucose metabolism and closely associate with liver cancer, the molecular mechanisms still remain to be elucidated. In this study, we attempt to understand the mechanism of insulin in promotion of liver cancer metabolism. We found that insulin increased pyruvate kinase M2 (PKM2) expression through reactive oxygen species (ROS) for regulating glucose consumption and lactate production, key process of glycolysis in hepatocellular carcinoma HepG2 and Bel7402 ce  ...[more]

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