Direct transfer of whole genomes from bacteria to yeast.
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ABSTRACT:
SUBMITTER: Karas BJ
PROVIDER: S-EPMC4034675 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Publications
Direct transfer of whole genomes from bacteria to yeast.
Karas Bogumil J BJ Jablanovic Jelena J Sun Lijie L Ma Li L Goldgof Gregory M GM Stam Jason J Ramon Adi A Manary Micah J MJ Winzeler Elizabeth A EA Venter J Craig JC Weyman Philip D PD Gibson Daniel G DG Glass John I JI Hutchison Clyde A CA Smith Hamilton O HO Suzuki Yo Y
Nature methods 20130331 5
Transfer of genomes into yeast facilitates genome engineering for genetically intractable organisms, but this process has been hampered by the need for cumbersome isolation of intact genomes before transfer. Here we demonstrate direct cell-to-cell transfer of bacterial genomes as large as 1.8 megabases (Mb) into yeast under conditions that promote cell fusion. Moreover, we discovered that removal of restriction endonucleases from donor bacteria resulted in the enhancement of genome transfer. ...[more]
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