Proteomics

Dataset Information

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Direct translation of incoming retroviral genomes


ABSTRACT: Viruses that carry a positive-sense, single-stranded (+ssRNA) RNA translate their genomes soon after entering the host cell to produce viral proteins, with the exception of retroviruses. A distinguishing feature of retroviruses is reverse transcription, where the +ssRNA genome serves as a template to synthesize a double-stranded DNA copy that subsequently integrates into the host genome. As retroviral RNAs are produced by the host cell transcriptional machinery and are largely indistinguishable from cellular mRNAs, we investigated the potential of incoming retroviral genomes to directly express proteins. Here we show through multiple, complementary methods that retroviral genomes are translated after entry. Our findings challenge the notion that retroviruses require reverse transcription to produce viral proteins. Synthesis of retroviral proteins in the absence of productive infection has significant implications for basic retrovirology, immune responses and gene therapy applications.

INSTRUMENT(S): Bruker Daltonics timsTOF series

ORGANISM(S): Homo Sapiens (human) Human Immunodeficiency Virus 1

SUBMITTER: Joerg Doellinger  

LAB HEAD: Peter Lasch

PROVIDER: PXD046777 | Pride | 2024-01-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Results.zip Other
checksum.txt Txt
combined_Human_HIV-gag-pol.fasta Fasta
ddaPASEF_30SPD_HIV_IP-gag_Mock_1_9-6-2023_2658.zip Other
ddaPASEF_30SPD_HIV_IP-gag_Mock_2_9-6-2023_2659.zip Other
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Publications

Direct translation of incoming retroviral genomes.

Köppke Julia J   Keller Luise-Elektra LE   Stuck Michelle M   Arnow Nicolas D ND   Bannert Norbert N   Doellinger Joerg J   Cingöz Oya O  

Nature communications 20240105 1


Viruses that carry a positive-sense, single-stranded (+ssRNA) RNA translate their genomes soon after entering the host cell to produce viral proteins, with the exception of retroviruses. A distinguishing feature of retroviruses is reverse transcription, where the +ssRNA genome serves as a template to synthesize a double-stranded DNA copy that subsequently integrates into the host genome. As retroviral RNAs are produced by the host cell transcriptional machinery and are largely indistinguishable  ...[more]

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