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Toward a functional annotation of the human genome using artificial transcription factors.

ABSTRACT: We have developed a novel, high-throughput approach to collecting randomly perturbed gene-expression profiles from the human genome.A human 293 cell library that stably expresses randomly chosen zinc-finger transcription factors was constructed, and the expression profile of each cell line was obtained using cDNA microarray technology.Gene expression profiles from a total of 132 cell lines were collected and analyzed by (1) a simple clustering method based on expression-profile similarity, and (2) the shortest-path analysis method. These analyses identified a number of gene groups, and further investigation revealed that the genes that were grouped together had close biological relationships. The artificial transcription factor-based random genome perturbation method thus provides a novel functional genomic tool for annotation and classification of genes in the human genome and those of many other organisms.

SUBMITTER: Lee DK

PROVIDER: S-EPMC403813 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature

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Toward a functional annotation of the human genome using artificial transcription factors.

Lee Dong-ki DK Park Jin Woo JW Kim Youn-Jae YJ Kim Jiwon J Lee Yangsoon Y Kim Jeonglim J Kim Jin-Soo JS

Genome research 20031201 12

We have developed a novel, high-throughput approach to collecting randomly perturbed gene-expression profiles from the human genome.A human 293 cell library that stably expresses randomly chosen zinc-finger transcription factors was constructed, and the expression profile of each cell line was obtained using cDNA microarray technology.Gene expression profiles from a total of 132 cell lines were collected and analyzed by (1) a simple clustering method based on expression-profile similarity, and ( ...[more]

PMID: 14656973

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