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DNA regions responsible for maintenance of Shingobium plasmid pYAN-2.

ABSTRACT: We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number small plasmid pYAN-2 (4,687 bp) from Sphingobium yanoikuyae. It does not encode any significant protein sequence and has one direct repeat for possible secondary structures. Moreover, a 70 bp fragment, upstream of the above sequence, completely stabilized the unstable pSC101 plasmid in E. coli.

SUBMITTER: Hayashi H

PROVIDER: S-EPMC4041236 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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DNA regions responsible for maintenance of Shingobium plasmid pYAN-2.

Hayashi Hiroe H Kurusu Yasurou Y

Microbes and environments 20140117 1

We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number small plasmid pYAN-2 (4,687 bp) from Sphingobium yanoikuyae. It does not encode any significant protein sequence and has one direct repeat for possible secondary structures. Moreover, a 70 bp fragment, upstream of the above sequence, completely stabiliz ...[more]

PMID: 24441514

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