Ontology highlight
ABSTRACT:
SUBMITTER: Goldberg JM
PROVIDER: S-EPMC4041396 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20131122 49
Previously we have shown that thioamides can be incorporated into proteins as minimally perturbing fluorescence-quenching probes to study protein dynamics, folding, and aggregation. Here, we show that the spontaneity of photoinduced electron transfer between a thioamide and an excited fluorophore is governed by the redox potentials of each moiety according to a Rehm-Weller-type model. We have used this model to predict thioamide quenching of various common fluorophores, and we rigorously tested ...[more]