Thioamide quenching of intrinsic protein fluorescence.
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ABSTRACT:
SUBMITTER: Goldberg JM
PROVIDER: S-EPMC7759719 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Publications
Thioamide quenching of intrinsic protein fluorescence.
Goldberg Jacob M JM Wissner Rebecca F RF Klein Alyssa M AM Petersson E James EJ
Chemical communications (Cambridge, England) 20110912 10
Thioamides quench tryptophan and tyrosine fluorescence in a distance-dependent manner and thus can be used to monitor the binding of thioamide-containing peptides to proteins. Since thioamide analogs of the natural amino acids can be synthetically incorporated into peptides, they can function as minimally-perturbing probes of protein/peptide interactions. ...[more]
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