Ontology highlight
ABSTRACT:
SUBMITTER: Neumann P
PROVIDER: S-EPMC4041423 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Neumann Piotr P Lakomek Kristina K Naumann Peter-Thomas PT Erwin Whitney M WM Lauhon Charles T CT Ficner Ralf R
Nucleic acids research 20140405 10
In prokaryotes and archaea transfer ribonucleic acid (tRNA) stability as well as cellular UV protection relies on the post-transcriptional modification of uracil at position 8 (U8) of tRNAs by the 4-thiouridine synthetase ThiI. Here, we report three crystal structures of ThiI from Thermotoga maritima in complex with a truncated tRNA. The RNA is mainly bound by the N-terminal ferredoxin-like domain (NFLD) and the THUMP domain of one subunit within the ThiI homo-dimer thereby positioning the U8 cl ...[more]