Unknown

Dataset Information

0

CDK2-dependent phosphorylation of Suv39H1 is involved in control of heterochromatin replication during cell cycle progression.


ABSTRACT: Although several studies have suggested that the functions of heterochromatin regulators may be regulated by post-translational modifications during cell cycle progression, regulation of the histone methyltransferase Suv39H1 is not fully understood. Here, we demonstrate a direct link between Suv39H1 phosphorylation and cell cycle progression. We show that CDK2 phosphorylates Suv39H1 at Ser391 and these phosphorylation levels oscillate during the cell cycle, peaking at S phase and maintained during S-G2-M phase. The CDK2-mediated phosphorylation of Suv39H1 at Ser391 results in preferential dissociation from chromatin. Furthermore, phosphorylation-mediated dissociation of Suv39H1 from chromatin causes an enhanced occupancy of JMJD2A histone demethylase on heterochromatin and alterations in inactive histone marks. Overexpression of phospho-mimic Suv39H1 induces early replication of heterochromatin, suggesting the importance of Suv39H1 phosphorylation in the replication of heterochromatin. Moreover, overexpression of phospho-defective Suv39H1 caused altered replication timing of heterochromatin and increases sensitivity to replication stress. Collectively, our data suggest that phosphorylation-mediated modulation of Suv39H1-chromatin association may be an initial step in heterochromatin replication.

SUBMITTER: Park SH 

PROVIDER: S-EPMC4041437 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

CDK2-dependent phosphorylation of Suv39H1 is involved in control of heterochromatin replication during cell cycle progression.

Park Su Hyung SH   Yu Seung Eun SE   Chai Young Gyu YG   Jang Yeun Kyu YK  

Nucleic acids research 20140411 10


Although several studies have suggested that the functions of heterochromatin regulators may be regulated by post-translational modifications during cell cycle progression, regulation of the histone methyltransferase Suv39H1 is not fully understood. Here, we demonstrate a direct link between Suv39H1 phosphorylation and cell cycle progression. We show that CDK2 phosphorylates Suv39H1 at Ser391 and these phosphorylation levels oscillate during the cell cycle, peaking at S phase and maintained duri  ...[more]

Similar Datasets

| S-EPMC5538822 | biostudies-literature
| S-EPMC3094048 | biostudies-literature
| S-EPMC3670391 | biostudies-literature
| S-EPMC3220541 | biostudies-literature
| S-EPMC5160415 | biostudies-other
| S-EPMC2642970 | biostudies-literature
| S-EPMC3778871 | biostudies-literature
| S-EPMC4861244 | biostudies-literature
| S-EPMC7108819 | biostudies-literature
| S-EPMC3865017 | biostudies-literature