Ontology highlight
ABSTRACT:
SUBMITTER: Ward M
PROVIDER: S-EPMC404402 | biostudies-literature | 2004 May
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20040501 5
Two different humanized immunoglobulin G1(kappa) antibodies and an Fab' fragment were produced by Aspergillus niger. The antibodies were secreted into the culture supernatant. Both light and heavy chains were initially synthesized as fusion proteins with native glucoamylase. After antibody assembly, cleavage by A. niger KexB protease allowed the release of free antibody. Purification by hydrophobic charge induction chromatography proved effective at removing any antibody to which glucoamylase re ...[more]