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Characterization of d-xylose reductase, XyrB, from Aspergillus niger.


ABSTRACT: d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).

SUBMITTER: Terebieniec A 

PROVIDER: S-EPMC8020424 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Characterization of d-xylose reductase, XyrB, from <i>Aspergillus niger</i>.

Terebieniec Agata A   Chroumpi Tania T   Dilokpimol Adiphol A   Aguilar-Pontes Maria Victoria MV   Mäkelä Miia R MR   de Vries Ronald P RP  

Biotechnology reports (Amsterdam, Netherlands) 20210315


d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from <i>Aspergillus niger</i>. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combina  ...[more]

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