Ontology highlight
ABSTRACT:
SUBMITTER: Pancera M
PROVIDER: S-EPMC4046252 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Pancera Marie M Shahzad-Ul-Hussan Syed S Doria-Rose Nicole A NA McLellan Jason S JS Bailer Robert T RT Dai Kaifan K Loesgen Sandra S Louder Mark K MK Staupe Ryan P RP Yang Yongping Y Zhang Baoshan B Parks Robert R Eudailey Joshua J Lloyd Krissey E KE Blinn Julie J Alam S Munir SM Haynes Barton F BF Amin Mohammed N MN Wang Lai-Xi LX Burton Dennis R DR Koff Wayne C WC Nabel Gary J GJ Mascola John R JR Bewley Carole A CA Kwong Peter D PD
Nature structural & molecular biology 20130526 7
HIV-1 uses a diverse N-linked-glycan shield to evade recognition by antibody. Select human antibodies, such as the clonally related PG9 and PG16, recognize glycopeptide epitopes in the HIV-1 V1-V2 region and penetrate this shield, but their ability to accommodate diverse glycans is unclear. Here we report the structure of antibody PG16 bound to a scaffolded V1-V2, showing an epitope comprising both high mannose-type and complex-type N-linked glycans. We combined structure, NMR and mutagenesis an ...[more]