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Amplification of coccidioidal DNA in clinical specimens by PCR.

ABSTRACT: Coccidioides DNA was amplified from serum by a PCR using coccidioid-specific primers. A 239-bp product was visualized when 10 fg of exogenous coccidioidal DNA was subjected to amplification. This product was demonstrated in some human and mouse sera prior to the detection of coccidioidal antibodies.

SUBMITTER: Johnson SM 

PROVIDER: S-EPMC404645 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature

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Amplification of coccidioidal DNA in clinical specimens by PCR.

Johnson Suzanne M SM   Simmons Keira A KA   Pappagianis Demosthenes D  

Journal of clinical microbiology 20040501 5

Coccidioides DNA was amplified from serum by a PCR using coccidioid-specific primers. A 239-bp product was visualized when 10 fg of exogenous coccidioidal DNA was subjected to amplification. This product was demonstrated in some human and mouse sera prior to the detection of coccidioidal antibodies. ...[more]

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