Unknown

Dataset Information

0

Autophagy correlates with maintenance of salivary gland function following radiation.


ABSTRACT: The current standard of care for head and neck cancer includes surgical resection of the tumor followed by targeted head and neck radiation. This radiotherapy results in a multitude of negative side effects in adjacent normal tissues. Autophagy is a cellular mechanism that could be targeted to ameliorate these side effects based on its role in cellular homeostasis. In this study, we utilized Atg5(f/f);Aqp5-Cre mice which harbor a conditional knockout of Atg5, in salivary acinar cells. These autophagy-deficient mice display increased radiosensitivity. Treatment of wild-type mice with radiation did not robustly induce autophagy following radiotherapy, however, using a model of preserved salivary gland function by IGF-1-treatment prior to irradiation, we demonstrate increased autophagosome formation 6-8?hours following radiation. Additionally, administration of IGF-1 to Atg5(f/f);Aqp5-Cre mice did not preserve physiological function. Thus, autophagy appears to play a beneficial role in salivary glands following radiation and pharmacological induction of autophagy could alleviate the negative side effects associated with therapy for head and neck cancer.

SUBMITTER: Morgan-Bathke M 

PROVIDER: S-EPMC4047540 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Autophagy correlates with maintenance of salivary gland function following radiation.

Morgan-Bathke Maria M   Hill Grace A GA   Harris Zoey I ZI   Lin Her H HH   Chibly Alex M AM   Klein Rob R RR   Burd Randy R   Ann David K DK   Limesand Kirsten H KH  

Scientific reports 20140606


The current standard of care for head and neck cancer includes surgical resection of the tumor followed by targeted head and neck radiation. This radiotherapy results in a multitude of negative side effects in adjacent normal tissues. Autophagy is a cellular mechanism that could be targeted to ameliorate these side effects based on its role in cellular homeostasis. In this study, we utilized Atg5(f/f);Aqp5-Cre mice which harbor a conditional knockout of Atg5, in salivary acinar cells. These auto  ...[more]

Similar Datasets

| S-EPMC4249875 | biostudies-literature
| S-EPMC7557086 | biostudies-literature
| S-EPMC6927217 | biostudies-literature
| S-EPMC9126227 | biostudies-literature
| S-EPMC3849572 | biostudies-literature
| S-EPMC10242138 | biostudies-literature
| S-EPMC10028956 | biostudies-literature
| S-EPMC7767137 | biostudies-literature
| S-EPMC7903845 | biostudies-literature
| S-EPMC7648631 | biostudies-literature