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Quantitative temporal viromics: an approach to investigate host-pathogen interaction.

ABSTRACT: A systematic quantitative analysis of temporal changes in host and viral proteins throughout the course of a productive infection could provide dynamic insights into virus-host interaction. We developed a proteomic technique called "quantitative temporal viromics" (QTV), which employs multiplexed tandem-mass-tag-based mass spectrometry. Human cytomegalovirus (HCMV) is not only an important pathogen but a paradigm of viral immune evasion. QTV detailed how HCMV orchestrates the expression of >8,000 cellular proteins, including 1,200 cell-surface proteins to manipulate signaling pathways and counterintrinsic, innate, and adaptive immune defenses. QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets. Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined. QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model.

SUBMITTER: Weekes MP

PROVIDER: S-EPMC4048463 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Quantitative temporal viromics: an approach to investigate host-pathogen interaction.

Weekes Michael P MP Tomasec Peter P Huttlin Edward L EL Fielding Ceri A CA Nusinow David D Stanton Richard J RJ Wang Eddie C Y ECY Aicheler Rebecca R Murrell Isa I Wilkinson Gavin W G GWG Lehner Paul J PJ Gygi Steven P SP

Cell 20140601 6

A systematic quantitative analysis of temporal changes in host and viral proteins throughout the course of a productive infection could provide dynamic insights into virus-host interaction. We developed a proteomic technique called "quantitative temporal viromics" (QTV), which employs multiplexed tandem-mass-tag-based mass spectrometry. Human cytomegalovirus (HCMV) is not only an important pathogen but a paradigm of viral immune evasion. QTV detailed how HCMV orchestrates the expression of >8,00 ...[more]

PMID: 24906157

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Quantitative temporal viromics: an approach to investigate host-pathogen interaction.

Publications

Quantitative temporal viromics: an approach to investigate host-pathogen interaction.

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