Ontology highlight
ABSTRACT:
SUBMITTER: Poh YC
PROVIDER: S-EPMC4050279 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Poh Yeh-Chuin YC Chen Junwei J Hong Ying Y Yi Haiying H Zhang Shuang S Chen Junjian J Wu Douglas C DC Wang Lili L Jia Qiong Q Singh Rishi R Yao Wenting W Tan Youhua Y Tajik Arash A Tanaka Tetsuya S TS Wang Ning N
Nature communications 20140530
Mammalian inner cell mass cells undergo lineage-specific differentiation into germ layers of endoderm, mesoderm and ectoderm during gastrulation. It has been a long-standing challenge in developmental biology to replicate these organized germ layer patterns in culture. Here we present a method of generating organized germ layers from a single mouse embryonic stem cell cultured in a soft fibrin matrix. Spatial organization of germ layers is regulated by cortical tension of the colony, matrix dime ...[more]