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Crystallization and preliminary X-ray diffraction analysis of Hypocrea jecorina Cel7A in two new crystal forms.


ABSTRACT: Cel7A (previously known as cellobiohydrolase I) from Hypocrea jecorina was crystallized in two crystalline forms, neither of which have been previously reported. Both forms co-crystallize under the same crystallization conditions. The first crystal form belonged to space group C2, with unit-cell parameters a=152.5, b=44.9, c=57.6?Å, ?=101.2°, and diffracted X-rays to 1.5?Å resolution. The second crystal form belonged to space group P6?22, with unit-cell parameters a=b?155, c?138?Å, and diffracted X-rays to 2.5?Å resolution. The crystals were obtained using full-length Cel7A, which consists of a large 434-residue N-terminal catalytic domain capable of cleaving cellulose, a 27-residue flexible linker and a small 36-residue C-terminal carbohydrate-binding module (CBM). However, a preliminary analysis of the electron-density maps suggests that the linker and CBM are disordered in both crystal forms. Complete refinement and structure analysis are currently in progress.

SUBMITTER: Bodenheimer AM 

PROVIDER: S-EPMC4051535 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray diffraction analysis of Hypocrea jecorina Cel7A in two new crystal forms.

Bodenheimer Annette M AM   Cuneo Matthew J MJ   Swartz Paul D PD   He Junhong J   O'Neill Hugh M HM   Myles Dean A A DA   Evans Barbara R BR   Meilleur Flora F  

Acta crystallographica. Section F, Structural biology communications 20140510 Pt 6


Cel7A (previously known as cellobiohydrolase I) from Hypocrea jecorina was crystallized in two crystalline forms, neither of which have been previously reported. Both forms co-crystallize under the same crystallization conditions. The first crystal form belonged to space group C2, with unit-cell parameters a=152.5, b=44.9, c=57.6 Å, β=101.2°, and diffracted X-rays to 1.5 Å resolution. The second crystal form belonged to space group P6₃22, with unit-cell parameters a=b≃155, c≃138 Å, and diffracte  ...[more]

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