Dataset Information

Secreted ?3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.

ABSTRACT: Integrins are a large family of heterodimeric proteins that are involved in cell adhesion, migration, and proliferation. Integrin diversity and function is regulated by alternative splicing. Membrane-bound and truncated ?3-integrins were shown to be key players in cancer metastasis. However, the immunomodulatory functions of the soluble (s) ?3-integrin have not been investigated yet. In this study, we described a novel form of s?3-integrin in acute myeloid leukaemia (AML) patients. Furthermore, we assessed the role of the s?3-integrin in the modulation of natural killer (NK)-cell activity. Levels of s?3-integrin were analysed in plasma samples of 23 AML patients and 26 healthy donors by ELISA. The capacity of s?3-integrin to regulate NK cell activity was investigated using proliferation, cytokine secretion, and cytotoxicity assays. Circulating s?3-integrin was detected in the plasma of 8 AML patients. NK cells showed significantly higher proliferation rates after stimulation with s?3-integrin and IL-2, IL-15 (73%). Significant increases in the NK cells' secreted levels of TNF-?, IFN-? were measured in presence of s?3-integrin. In addition, s?3-integrin caused the upregulation of Granzyme B transcripts levels as well as FasL expression levels in NK cells. Most importantly, significantly higher K562 or AML blast target cell lysis rates were observed when NK cells were exposed to s?3-integrin. This study reports the identification of a novel s?3-integrin in AML patients and provides novel insights into its role in the immunomodulation of NK cell activity.

SUBMITTER: Skaik Y

PROVIDER: S-EPMC4053493 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.

Skaik Younis Y Vahlsing Stefanie S Goudeva Lilia L Eiz-Vesper Britta B Battermann Anja A Blasczyk Rainer R Figueiredo Constança C

PloS one 20140611 2

Integrins are a large family of heterodimeric proteins that are involved in cell adhesion, migration, and proliferation. Integrin diversity and function is regulated by alternative splicing. Membrane-bound and truncated β3-integrins were shown to be key players in cancer metastasis. However, the immunomodulatory functions of the soluble (s) β3-integrin have not been investigated yet. In this study, we described a novel form of sβ3-integrin in acute myeloid leukaemia (AML) patients. Furthermore, ...[more]

PMID: 24919191

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Project description:Background Acute myeloid leukemia (AML) is the most common type of acute leukemia in adults. Given the high relapse rate, more effective treatments are needed to improve clinical outcomes. We previously demonstrated that heme oxygenase 1 (HO1) is overexpressed in AML, while the functional roles of HO1 remain unclear. Methods Bioinformatics analysis and flow cytometry were conducted to assess the association between HO1 levels and immune cells or immune checkpoint/ligand molecules in AML patients. Primary natural killer (NK) cells were purified and subsequently co-cultured in vitro with transduced AML cells to determine the effects of HO1 expression on NK cell functions. AML mice models were established to investigate the effects of HO1 expression on cytotoxic effects of NK cells in vivo. The molecular mechanism was studied by flow cytometry, quantitative real-time PCR (qRT-PCR), western blotting, and immunoprecipitation. Results Bioinformatics analysis indicated a correlation between HO1 expression and the AML immune microenvironment. The present study findings indicated that HO1 specifically downregulates the expression of CD48, a ligand of the NK cell-activating receptor 2B4, thus decreasing the cytotoxic effect of NK cells. HO1 overexpression promoted tumor growth and inhibited the cytotoxic effect of NK cells in the AML mice model. Mechanistic investigations found that HO1 directly interacted with Sirt1 and increased its expression and deacetylase activity. With the overexpression of HO1, increased Sirt1 in AML cells enabled histone H3K27 deacetylation to suppress CD48 transcription and expression. Administration of Sirt1 inhibitor restored the expression of CD48. Conclusions Collectively, HO1 promotes NK cell dysfunction in AML. Therefore, restoring NK cell function by inhibiting HO1 activity is a potential immunotherapeutic approach against AML. Supplementary Information The online version contains supplementary material available at 10.1186/s12967-022-03589-z.

Dataset Information

Secreted ?3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.

Publications

Secreted β3-integrin enhances natural killer cell activity against acute myeloid leukemia cells.

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