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24S,25-Epoxycholesterol in mouse and rat brain.


ABSTRACT: 24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4?g/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20?g/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7?-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide.

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4053837 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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24S,25-Epoxycholesterol in mouse and rat brain.

Wang Yuchen Y   Karu Kersti K   Meljon Anna A   Turton John J   Yau Joyce L JL   Seckl Jonathan R JR   Wang Yuqin Y   Griffiths William J WJ  

Biochemical and biophysical research communications 20140514 2


24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of  ...[more]

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