Protein-protein interactions leading to recruitment of the host DNA sliding clamp by the hyperthermophilic Sulfolobus islandicus rod-shaped virus 2.
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ABSTRACT:
SUBMITTER: Gardner AF
PROVIDER: S-EPMC4054388 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
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Protein-protein interactions leading to recruitment of the host DNA sliding clamp by the hyperthermophilic Sulfolobus islandicus rod-shaped virus 2.
Gardner Andrew F AF Bell Stephen D SD White Malcolm F MF Prangishvili David D Krupovic Mart M
Journal of virology 20140402 12
Viruses infecting hyperthermophilic archaea typically do not encode DNA polymerases, raising questions regarding their genome replication. Here, using a yeast two-hybrid approach, we have assessed interactions between proteins of Sulfolobus islandicus rod-shaped virus 2 (SIRV2) and the host-encoded proliferating cell nuclear antigen (PCNA), a key DNA replication protein in archaea. Five SIRV2 proteins were found to interact with PCNA, providing insights into the recruitment of host replisome for ...[more]
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