Ontology highlight
ABSTRACT:
SUBMITTER: Gaspar-Maia A
PROVIDER: S-EPMC4055026 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Gaspar-Maia Alexandre A Qadeer Zulekha A ZA Hasson Dan D Ratnakumar Kajan K Leu N Adrian NA Leroy Gary G Liu Shichong S Costanzi Carl C Valle-Garcia David D Schaniel Christoph C Lemischka Ihor I Garcia Benjamin B Pehrson John R JR Bernstein Emily E
Nature communications 20130101
The chromatin template imposes an epigenetic barrier during the process of somatic cell reprogramming. Using fibroblasts derived from macroH2A double knockout (dKO) mice, here we show that these histone variants act cooperatively as a barrier to induced pluripotency. Through manipulation of macroH2A isoforms, we further demonstrate that macroH2A2 is the predominant barrier to reprogramming. Genomic analyses reveal that macroH2A1 and macroH2A2, together with H3K27me3, co-occupy pluripotency genes ...[more]