Genome-wide binding of MBD2 reveals strong preference for highly methylated loci.
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ABSTRACT:
SUBMITTER: Menafra R
PROVIDER: S-EPMC4057170 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
Genome-wide binding of MBD2 reveals strong preference for highly methylated loci.
Menafra Roberta R Brinkman Arie B AB Matarese Filomena F Franci Gianluigi G Bartels Stefanie J J SJ Nguyen Luan L Shimbo Takashi T Wade Paul A PA Hubner Nina C NC Stunnenberg Hendrik G HG
PloS one 20140613 6
MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. In this study we adopted an MBD2 tagging-approach to study its genome wide binding characteristics. We show that in vivo MBD2 is mainly recruited to CpG island promoters that are highly methylated. Interestingly, MBD2 binds around 1 kb downstream of the transcription start site of a subset of ∼ 400 CpG island promoters that are characterized by the presence of act ...[more]
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