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Structural insights into FRS2? PTB domain recognition by neurotrophin receptor TrkB.

ABSTRACT: The fibroblast growth factor receptor (FGFR) substrate 2 (FRS2) family proteins function as scaffolding adapters for receptor tyrosine kinases (RTKs). The FRS2? proteins interact with RTKs through the phosphotyrosine-binding (PTB) domain and transfer signals from the activated receptors to downstream effector proteins. Here, we report the nuclear magnetic resonance structure of the FRS2? PTB domain bound to phosphorylated TrkB. The structure reveals that the FRS2?-PTB domain is comprised of two distinct but adjacent pockets for its mutually exclusive interaction with either nonphosphorylated juxtamembrane region of the FGFR, or tyrosine phosphorylated peptides TrkA and TrkB. The new structural insights suggest rational design of selective small molecules through targeting of the two conjunct pockets in the FRS2? PTB domain.

SUBMITTER: Zeng L

PROVIDER: S-EPMC4057350 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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Structural insights into FRS2α PTB domain recognition by neurotrophin receptor TrkB.

Zeng Lei L Kuti Miklos M Mujtaba Shiraz S Zhou Ming-Ming MM

Proteins 20140701 7

The fibroblast growth factor receptor (FGFR) substrate 2 (FRS2) family proteins function as scaffolding adapters for receptor tyrosine kinases (RTKs). The FRS2α proteins interact with RTKs through the phosphotyrosine-binding (PTB) domain and transfer signals from the activated receptors to downstream effector proteins. Here, we report the nuclear magnetic resonance structure of the FRS2α PTB domain bound to phosphorylated TrkB. The structure reveals that the FRS2α-PTB domain is comprised of two ...[more]

PMID: 24470253

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