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Recombinant dense granular protein (GRA5) for detection of human toxoplasmosis by Western blot.


ABSTRACT: Toxoplasma gondii infects all warm-blooded animals, including humans, causing serious public health problems and great economic loss for the food industry. Commonly used serological tests require costly and hazardous preparation of whole Toxoplasma lysate antigens from tachyzoites. Here, we have evaluated an alternative method for antigen production, which involved a prokaryotic expression system. Specifically, we expressed T. gondii dense granular protein-5 (GRA5) in Escherichia coli and isolated it by affinity purification. The serodiagnostic potential of the purified recombinant GRA5 (rGRA5) was tested through Western blot analysis against 212 human patient serum samples. We found that rGRA5 protein was 100% specific for analysis of toxoplasmosis-negative human sera. Also, rGRA5 was able to detect acute and chronic T. gondii infections (sensitivities of 46.8% and 61.2%, resp.).

SUBMITTER: Ching XT 

PROVIDER: S-EPMC4058840 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Recombinant dense granular protein (GRA5) for detection of human toxoplasmosis by Western blot.

Ching Xiao Teng XT   Lau Yee Ling YL   Fong Mun Yik MY   Nissapatorn Veeranoot V   Andiappan Hemah H  

BioMed research international 20140529


Toxoplasma gondii infects all warm-blooded animals, including humans, causing serious public health problems and great economic loss for the food industry. Commonly used serological tests require costly and hazardous preparation of whole Toxoplasma lysate antigens from tachyzoites. Here, we have evaluated an alternative method for antigen production, which involved a prokaryotic expression system. Specifically, we expressed T. gondii dense granular protein-5 (GRA5) in Escherichia coli and isolat  ...[more]

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