Ontology highlight
ABSTRACT:
SUBMITTER: Smirnova I
PROVIDER: S-EPMC4060661 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Smirnova Irina I Kasho Vladimir V Kaback H Ronald HR
Proceedings of the National Academy of Sciences of the United States of America 20140528 23
Galactoside/H(+) symport across the cytoplasmic membrane of Escherichia coli is catalyzed by lactose permease (LacY), which uses an alternating access mechanism with opening and closing of deep cavities on the periplasmic and cytoplasmic sides. In this study, conformational changes in LacY initiated by galactoside binding were monitored in real time by Trp quenching/unquenching of bimane, a small fluorophore covalently attached to the protein. Rates of change in bimane fluorescence on either sid ...[more]