Dataset Information

Plasmid-mediated AmpC ?-lactamase (CMY-2) gene in Salmonella typhimurium isolated from diarrheic pigs in South Korea.

ABSTRACT: BACKGROUND: Salmonella resistant to third-generation cephalosporin has been isolated from an increasing number of animals worldwide. The purpose of this study was to examine ESBL (extended-spectrum ?-lactamases)-producing and PABL (plasmid-mediated AmpC ?-lactamases)-producing Salmonella isolates from pigs in South Korea. RESULTS: Salmonella Typhimurium KVCC-BA1300259 was resistant to ampicillin, amoxicillin/clavulanic acid, cephalothin, chloramphenicol, florfenicol, cefoxithin, gentamicin, nalidixic acid, trimethoprim/sulfamethoxazole, tetracycline, and ceftiofur. The results of a double-disk synergy test and PCR confirmed that the isolate produced CMY-2 (PABL). Analysis of plasmid incompatibility (Inc) groups revealed the presence of IncA/C and IncFIB, indicating antimicrobial resistance. This study is the first to identify S. Typhimurium isolates harboring CMY-2 in pigs in South Korea. CONCLUSIONS: The presence of CMY-2 in pigs poses a significant threat of possible horizontal spread between animals and humans.

SUBMITTER: Lee KE

PROVIDER: S-EPMC4060855 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Publications

Plasmid-mediated AmpC β-lactamase (CMY-2) gene in Salmonella typhimurium isolated from diarrheic pigs in South Korea.

Lee Ki-Eun KE Lim Seong-In SI Choi Hwan-Won HW Lim Suk-Kyung SK Song Jae-Young JY An Dong-Jun DJ

BMC research notes 20140602

<h4>Background</h4>Salmonella resistant to third-generation cephalosporin has been isolated from an increasing number of animals worldwide. The purpose of this study was to examine ESBL (extended-spectrum β-lactamases)-producing and PABL (plasmid-mediated AmpC β-lactamases)-producing Salmonella isolates from pigs in South Korea.<h4>Results</h4>Salmonella Typhimurium KVCC-BA1300259 was resistant to ampicillin, amoxicillin/clavulanic acid, cephalothin, chloramphenicol, florfenicol, cefoxithin, gen ...[more]

PMID: 24885386

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Project description:Vibrio parahaemolyticus is an important causative agent of gastroenteritis, with the consumption of contaminated seafood being the major transmission route. Resistance to penicillin is common among V. parahaemolyticus strains, whereas cephalosporin resistance remains rare. In an attempt to assess the current prevalence and characteristics of antibiotic resistance of this pathogen in common food samples, a total of 54 (17% of the total samples) V. parahaemolyticus strains were isolated from 318 meat and seafood samples purchased from supermarkets and wet markets in Shenzhen, China, in 2013. These isolates exhibited high-level resistance to ampicillin, yet they were mostly susceptible to other antimicrobials, except for two that were resistant to extended-spectrum cephalosporins. The ?-lactamase gene blaPER-1 was detectable in one strain, V. parahaemolyticus V43, which was resistant to both third- and fourth-generation cephalosporins. Compared to other blaPER-1-positive V. parahaemolyticus strains reported in our previous studies, strain V43 was found to harbor an ?200-kb conjugative plasmid carrying genes that were different from the antimicrobial resistance genes reported from the previous studies. The ?-lactamase gene blaCMY-2 was detectable for the first time in another V. parahaemolyticus isolate, V4, which was resistant to third-generation cephalosporins. This blaCMY-2 gene was shown to be located in an ?150-kb IncA/C-type conjugative plasmid with a genetic structure consisting of traB-traV-traA-ISEcp1-blaCMY-2-blc-sugE-encR-orf1-orf2-orf3-orf4-dsbC-traC, which is identical to that of other IncA/C conjugative plasmids in Enterobacteriaceae, albeit with a different size. These findings indicate that the transmission of extended-spectrum-?-lactamase (ESBL) and AmpC ?-lactamase genes via conjugative plasmids can mediate the development of extended-spectrum cephalosporin resistance in V. parahaemolyticus, thereby posing a potential threat to public health.

Dataset Information

Plasmid-mediated AmpC ?-lactamase (CMY-2) gene in Salmonella typhimurium isolated from diarrheic pigs in South Korea.

Publications

Plasmid-mediated AmpC β-lactamase (CMY-2) gene in Salmonella typhimurium isolated from diarrheic pigs in South Korea.

Similar Datasets

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