Ontology highlight
ABSTRACT:
SUBMITTER: Frohlich RF
PROVIDER: S-EPMC4067056 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Fröhlich Richard F G RF Schrank Evelyne E Zangger Klaus K
Carbohydrate research 20120825
A synthetic route to a trifluoromethyl and thiol containing glucose derivative (2,2,2-trifluoroethyl 6-thio-β-D-glucopyranoside) is presented, which is based on microwave-assisted Fischer glycosylation under increased pressure. This water-soluble, neutral thiol-compound can be used to selectively introduce a fluorine probe for (19)F NMR spectroscopy on cysteines in proteins. It can be attached under mild conditions in an aqueous environment without the risk of denaturing the protein. This tag ha ...[more]