Ontology highlight
ABSTRACT:
SUBMITTER: Joo JY
PROVIDER: S-EPMC4067617 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Joo Jin Young JY Lee Jonghwan J Ko Hae Young HY Lee Yong Seung YS Lim Do-Hwan DH Kim Eun-Young EY Cho Sujeong S Hong Ki-Sung KS Ko Jung Jae JJ Lee Suman S Lee Young Sik YS Choi Youngsok Y Lee Kyung-Ah KA Kim Soonhag S
Scientific reports 20140624
The development of gene delivery systems into embryos is challenging due to technical difficulties, delivery efficiency and toxicity. Here, we developed an organic compound (VisuFect)-mediated gene delivery system for zygotes. The VisuFect, which is hydrophilic and Cy5.5-labeled, was conjugated with poly(A) oligo (VFA). The VFA into CHO cells showed clathrin-mediated internalization and no toxicity. The VFA successfully penetrated through the zona pellucida of fertilized eggs of various species ...[more]