Freezing-induced perturbation of tertiary structure of a monoclonal antibody.
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ABSTRACT:
SUBMITTER: Liu L
PROVIDER: S-EPMC4069245 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Publications
Freezing-induced perturbation of tertiary structure of a monoclonal antibody.
Liu Lu L Braun Latoya Jones LJ Wang Wei W Randolph Theodore W TW Carpenter John F JF
Journal of pharmaceutical sciences 20140515 7
We studied the effects of pH and solution additives on freezing-induced perturbations in the tertiary structure of a monoclonal antibody (mAb) by intrinsic tryptophan fluorescence spectroscopy. In general, freezing caused perturbations in the tertiary structure of the mAb, which were reversible or irreversible depending on the pH or excipients present in the formulation. Protein aggregation occurred in freeze-thawed samples in which perturbations of the tertiary structure were observed, but the ...[more]
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