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Comprehensive phylogenetic diversity of [FeFe]-hydrogenase genes in termite gut microbiota.

ABSTRACT: Phylogenetic diversity of [FeFe]-hydrogenase (HydA) in termite guts was assessed by pyrosequencing PCR amplicons obtained using newly designed primers. Of 8,066 reads, 776 hydA phylotypes, defined with 97% nucleotide sequence identity, were recovered from the gut homogenates of three termite species, Hodotermopsis sjoestedti, Reticulitermes speratus, and Nasutitermes takasagoensis. The phylotype coverage was 92-98%, and the majority shared only low identity with database sequences. It was estimated that 194-745 hydA phylotypes existed in the gut of each termite species. Our results demonstrate that hydA gene diversity in the termite gut microbiota is much higher than previously estimated.

SUBMITTER: Zheng H

PROVIDER: S-EPMC4070709 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Comprehensive phylogenetic diversity of [FeFe]-hydrogenase genes in termite gut microbiota.

Zheng Hao H Bodington Dylan D Zhang Chong C Miyanaga Kazuhiko K Tanji Yasunori Y Hongoh Yuichi Y Xing Xin-Hui XH

Microbes and environments 20131115 4

Phylogenetic diversity of [FeFe]-hydrogenase (HydA) in termite guts was assessed by pyrosequencing PCR amplicons obtained using newly designed primers. Of 8,066 reads, 776 hydA phylotypes, defined with 97% nucleotide sequence identity, were recovered from the gut homogenates of three termite species, Hodotermopsis sjoestedti, Reticulitermes speratus, and Nasutitermes takasagoensis. The phylotype coverage was 92-98%, and the majority shared only low identity with database sequences. It was estima ...[more]

PMID: 24240187

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