Single-step selection of bivalent aptamers validated by comparison with SELEX using high-throughput sequencing.
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ABSTRACT:
SUBMITTER: Wilson R
PROVIDER: S-EPMC4070925 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
Single-step selection of bivalent aptamers validated by comparison with SELEX using high-throughput sequencing.
Wilson Robert R Bourne Christian C Chaudhuri Roy R RR Gregory Richard R Kenny John J Cossins Andrew A
PloS one 20140625 6
The identification of nucleic acid aptamers would be advanced if they could be obtained after fewer rounds of selection and amplification. In this paper the identification of bivalent aptamers for thrombin by SELEX and single-step selection are compared using next generation sequencing and motif finding informatics. Results show that similar aptamers are identified by both methods. This is significant because it shows that next generation sequencing and motif finding informatics have the potenti ...[more]
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