Ontology highlight
ABSTRACT:
SUBMITTER: Cho M
PROVIDER: S-EPMC2932614 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Cho Minseon M Xiao Yi Y Nie Jeff J Stewart Ron R Csordas Andrew T AT Oh Seung Soo SS Thomson James A JA Soh H Tom HT
Proceedings of the National Academy of Sciences of the United States of America 20100812 35
We describe the integration of microfluidic selection with high-throughput DNA sequencing technology for rapid and efficient discovery of nucleic acid aptamers. The Quantitative Selection of Aptamers through Sequencing method tracks the copy number and enrichment-fold of more than 10 million individual sequences through multiple selection rounds, enabling the identification of high-affinity aptamers without the need for the pool to fully converge to a small number of sequences. Importantly, this ...[more]