Ontology highlight
ABSTRACT:
SUBMITTER: Westmeyer GG
PROVIDER: S-EPMC4073233 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Westmeyer Gil G GG Emer Yelena Y Lintelmann Jutta J Jasanoff Alan A
Chemistry & biology 20140306 3
The ability to map patterns of gene expression noninvasively in living animals could have impact in many areas of biology. Reporter systems compatible with MRI could be particularly valuable, but existing strategies tend to lack sensitivity or specificity. Here we address the challenge of MRI-based gene mapping using the reporter enzyme secreted alkaline phosphatase (SEAP), in conjunction with a water-soluble metalloporphyrin contrast agent. SEAP cleaves the porphyrin into an insoluble product t ...[more]