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A new signal sequence trap using alkaline phosphatase as a reporter.

ABSTRACT: Secreted and transmembrane proteins are critical to the cell-cell interactions governing normal development and carcinogenesis. To facilitate the identification of such molecules, we have developed a novel signal sequence trap that uses human placental alkaline phosphatase as a reporter. Libraries from mouse prostate and human prostatic carcinoma were constructed to test the PST (peptide signal trap) system, resulting in the identification of several secreted and transmembrane proteins.

SUBMITTER: Chen H

PROVIDER: S-EPMC148306 | biostudies-other | 1999 Feb

REPOSITORIES: biostudies-other

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A new signal sequence trap using alkaline phosphatase as a reporter.

Chen H H Leder P P

Nucleic acids research 19990201 4

Secreted and transmembrane proteins are critical to the cell-cell interactions governing normal development and carcinogenesis. To facilitate the identification of such molecules, we have developed a novel signal sequence trap that uses human placental alkaline phosphatase as a reporter. Libraries from mouse prostate and human prostatic carcinoma were constructed to test the PST (peptide signal trap) system, resulting in the identification of several secreted and transmembrane proteins. ...[more]

PMID: 9927759

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