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Tyrosinase inhibitory activity of a glucosylated hydroxystilbene in mouse melan-a melanocytes.

ABSTRACT: 2,3,5,4'-Tetrahydroxystilbene-2-O-?-D-glucopyranoside (1), isolated from Polygonum multiflorum, is a noncompetitive inhibitor of tyrosinase in cell-free kinetics; it reduced the Vmax values in a dose-dependent manner. Compound 1 inhibited PKA-induced melanogenesis, reduced the protein expression of tyrosinase and its transcription factor, the microphthalmia-associated transcription factor, and lowered the complex formation between tyrosinase and tyrosinase-related protein 1 (TRP-1). Immunofluorescence microscopy revealed no association of tyrosinase with the endoplasmic reticulum or lysosomes, implying the absence of a direct effect of 1 on the maturation process of the enzyme. The antimelanogenic activity of 1 is likely mediated through a noncompetitive inhibition on tyrosinase, down-regulation of the expression of melanogenic proteins, and reduction of tyrosinase/TRP-1 complex formation.

SUBMITTER: Cheung FW

PROVIDER: S-EPMC4076036 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Tyrosinase inhibitory activity of a glucosylated hydroxystilbene in mouse melan-a melanocytes.

Cheung Florence Wing-Ki FW Leung Albert Wing-Nang AW Liu Wing Keung WK Che Chun-Tao CT

Journal of natural products 20140616 6

2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucopyranoside (1), isolated from Polygonum multiflorum, is a noncompetitive inhibitor of tyrosinase in cell-free kinetics; it reduced the Vmax values in a dose-dependent manner. Compound 1 inhibited PKA-induced melanogenesis, reduced the protein expression of tyrosinase and its transcription factor, the microphthalmia-associated transcription factor, and lowered the complex formation between tyrosinase and tyrosinase-related protein 1 (TRP-1). Immunofluore ...[more]

PMID: 24933607

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